Journal: The FASEB Journal

Article Title: Cardiac CapZ Regulation During Acute Exercise in Female Mice

doi: 10.1096/fj.202502431R

Figure Lengend Snippet: Cardiac CapZ regulation following acute exercise. Cardiac myofilaments were isolated after an acute bout of exercise and probed with immunoblotting. (A) Myofilament CapZ levels were not impacted by an acute bout of swimming. (B) CapZIP increased 51% ± 8% over control samples. (C) Phosphorylation of CapZIP at S179 decreased by 35% ± 5% compared to non‐exercise controls. (D) Myofilament‐associated Hsc70 levels were not altered by exercise. (E) BAG3 decreased by 12% ± 2% compared to controls. N = 9 in each group. Data are presented as mean ± SEM. * p < 0.05 versus control.

Article Snippet: Membranes were blocked in TBS containing 5% milk powder for 1 h at room temperature and then incubated with primary antibodies for CapZIP, phosphorylated CapZIP (S179), BAG3, PKC‐ε, actin (MilliporeSigma, Oakville, ON, Canada); Hsc70, leiomodin 2 (Santa Cruz Biotechnology, Dallas, TX, USA); PKC‐α, telethonin/Tcap (BD Biosciences, San Jose, CA, USA); tropomodulin 1 (Abcam Inc., Toronto, ON, Canada); and CapZ (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA) overnight at 4°C.

Techniques: Isolation, Western Blot, Control, Phospho-proteomics

Journal: The FASEB Journal

Article Title: Cardiac CapZ Regulation During Acute Exercise in Female Mice

doi: 10.1096/fj.202502431R

Figure Lengend Snippet: Cardiac CapZ‐deficient mice exhibit an impaired CapZ regulatory response to exercise. Cardiac myofilaments were isolated after an acute bout of exercise and probed with immunoblotting. Neither (A) CapZ, (B) CapZIP, (C) phosphorylation of CapZIP at S179, nor (D) myofilament‐associated Hsc70 levels were affected by exercise. (E) BAG3 increased by 10% ± 3% compared to controls. N = 9 in each group. Data are presented as mean ± SEM. * p < 0.05 versus control.

Article Snippet: Membranes were blocked in TBS containing 5% milk powder for 1 h at room temperature and then incubated with primary antibodies for CapZIP, phosphorylated CapZIP (S179), BAG3, PKC‐ε, actin (MilliporeSigma, Oakville, ON, Canada); Hsc70, leiomodin 2 (Santa Cruz Biotechnology, Dallas, TX, USA); PKC‐α, telethonin/Tcap (BD Biosciences, San Jose, CA, USA); tropomodulin 1 (Abcam Inc., Toronto, ON, Canada); and CapZ (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA) overnight at 4°C.

Techniques: Isolation, Western Blot, Phospho-proteomics, Control